Summary
Type: TLC
Application #: 400720
Catalog#: 1624026 - Silica G TLC Plates, polyester backed, 200um, 20x20cm 25/pk
Detection: differential staining by immersion in aqueous thionine, TLC scanner
Conditions: Eluent: chloroform / methanol / water (140:49:5.5, v/v), 1 h chamber
saturation minimum
Migration: 10 cm / 45 min
Sample volume: 10 ul of a 100 mg sample in 3 ml solvent
Authors: Hack H
Source: J Planar Chromatog 4 (1991) 80 - 84
Details
Legend: TOP TLC:
Samples:
1. Control extract chicken heart
2. Control extract shark heart
3. CL following incubation with pig pancreas PLA1 (phospholipase A)
4. Control extract pig heart
5. Pig heart, i